硕士导师

干细胞与再生医学

    现任广东省医学会细胞治疗学分会青年委员会委员,广东省中医药学会整合生殖医学专业委员会委员,干细胞与组织工程教育部重点实验室研究人员,广东省生殖医学重点实验室研究人员。主要研究成果包括:利用基因编辑技术建立多种人类遗传疾病灵长类动物模型,包括MCPH1基因缺失小脑症食蟹猴模型、FAH基因缺失酪氨酸血症I型食蟹猴模型及SHANK3基因缺失自闭症食蟹猴模型等,首次证实利用TALEN技术可以获得具有显著表型的食蟹猴模型;建立了多种转基因小鼠及人类的正常和疾病多能干细胞系;首次证实CX43能促进细胞重编程效率,为提高iPSC的形成效率及探讨细胞重编程机制提供重要的理论基础等。以第一作者在Cell Research、Hum Mol Genet等杂志发表论文多篇,主持参与国家重点研发项目、国家/省自然科学基金项目及广州市健康医疗协同创新重大专项等基金项目。

 

已发表SCI论文

  1. Ke Q#, Li L#, Yao X#, Lai X, Cai B, Chen H, Chen R, Zhai Z, Huang L, Li K, Hu A, Mao FF, Xiang AP, Tao L*, Li W*. Enhanced generation of human induced pluripotent stem cells by ectopic expression of Connexin 45. Sci Rep. 2017. Mar 28;7(1): p. 458. (IF:4.259)
  2. Ke Q#, Li W#, Lai X#, Chen H, Huang L, Kang Z, Li K, Ren J, Lin X, Zheng H, Huang W, Ma Y, Xu D, Chen Z, Song X, Lin X, Zhuang M, Wang T, Zhuang F, Xi J, Mao FF, Xia H, Lahn BT, Zhou Q*, Yang S*, Xiang AP*. TALEN-based generation of a cynomolgus monkey disease model for human microcephaly. Cell Res. 2016 Sep;26(9):1048-61. (IF:15.606)
  3. Ma Y#, Li J#, Wang G#, Ke Q#, Qiu S, Gao L, Wan H, Zhou Y, Xiang A P, Huang Q, Feng G*, Zhou Q*, Yang S*. Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies. Sci Rep, 2016 May 13. 6: p. 25888. (IF:4.259)
  4. Ke Q#, Li L#, Cai B#, Liu C, Yang Y, Gao Y, Huang WJ, Yuan XF, Wang T, Zhang Q, Harris AL, Tao L, Xiang AP*. Connexin 43 is involved in the generation of human induced pluripotent stem cells. Hum Mol Genet. 2013 Jun 1;22(11):2221-33. (IF:5.340)
  5. Cheng F#, Ke Q#, Chen F#, Cai B, Gao Y, Ye C, Wang D, Zhang L, Lahn BT, Li W, Xiang AP*. Protecting against wayward human induced pluripotent stem cells with a suicide gene. Biomaterials. 2012 Apr;33(11):3195-204. (IF:8.402)
  6. Zhang Y#, Wang J#, Huang W#, Cai J#, Ba J, Wang Y, Ke Q, Huang Y, Liu X, Qiu Y, Lu Q, Sui X, Shi Y, Wang T, Shen, H, Guan Y, Zhou Y, Chen Y, Wang M, Xiang AP*. Nuclear Nestin deficiency drives tumor senescence via lamin A/C-dependent nuclear deformation. Nature Communications, 2018. 9(1). (IF:12.353)
  7. Wang J#, Liu X#, Qiu Y#, Shi Y, Cai J, Wang B, Wei X, Ke Q, Sui X, Wang Y, Huang Y, Li H, Wang T, Lin R, Liu Q, Xiang AP*. Cell adhesion-mediated mitochondria transfer contributes to mesenchymal stem cell-induced chemoresistance on T cell acute lymphoblastic leukemia cells. J Hematol Oncol. 2018 Jan 22;11(1):11. (IF:6.350)
  8. Wang J#, Huang Y#, Cai J#, Ke Q, Xiao J, Huang W, Li H, Qiu Y, Wang Y, Zhang B, Wu H, Zhang Y, Sui X, Bardeesi ASA, Xiang AP*. A Nestin-Cyclin-Dependent Kinase 5-Dynamin-Related Protein 1 Axis Regulates Neural Stem/Progenitor Cell Stemness via a Metabolic Shift. Stem Cells. 2018 Jan 3. doi: 10.1002/stem.2769. (IF:5.599)
  9. Zhang C, Liao W, Cai B, Liu F, Ke Q, Zhu X, He X, Hu A*. The similarities between smDCs and regDCs in alleviating the immune injury caused by transplantation of hepatocytes differentiated from ESCs, Stem Cell Res Ther, 2017.11.21, 8(1): 266~266. (IF:4.211)
  10. Li W#, Huang L#, Zeng J#, Lin W, Li K, Sun J, Huang W, Chen J, Wang G, Ke Q, Duan J, Lai X, Chen R, Liu M, Liu Y, Wang T, Yang X, Chen Y, Xia H*, Xiang AP*. Characterization and transplantation of enteric neural crest cells from human induced pluripotent stem cells. Mol Psychiatry. 2016 Oct 25. doi: 10.1038/mp.2016.191. (IF:13.204)
  11. Wang J#, Cai J#, Huang Y#, Ke Q, Wu B, Wang S, Han X, Wang T, Wang Y, Li W, Lao C, Song W, Xiang AP*. Nestin regulates proliferation and invasion of gastrointestinal stromal tumor cells by altering mitochondrial dynamics.  Oncogene. 2016 Jun 16;35(24):3139-50. (IF:7.519)
  12. Li W#, Huang L#, Lin W#, Ke Q, Chen R, Lai X, Wang X, Zhang J, Jiang M, Huang W, Wang T, Yang X, Chen Y, Song W*, Xiang AP*. Engraftable neural crest stem cells derived from cynomolgus monkey embryonic stem cells. Biomaterials. 2015 Jan;39:75-84. (IF:8.402)

 

获得科研基金项目

  1. 国自然青年科学基金项目 31301219(2014.01-2016.12)项目负责人

       题目:CX43 影响诱导多能干细胞形成的机制研究

  1. 中山大学重大项目培育和新兴 、交叉学科资助计划项目 15ykjc01c(2015.10-2017.10)项目负责人

      题目:MCPH1基因敲除食蟹猴模型的构建及该基因对神经分化的作用

  1. 教育部博士点基金项目 20130171120061(2014.01-2016.12)项目负责人

     题目:缝隙连接蛋白对诱导多能干细胞形成的影响及相关机制

  1. 国家重点研发计划项目 2018YFA0107200(2018.07-2022.12)课题骨干

     题目:间充质干细胞亚群的功能鉴定、分离制备与疗效评估

  1. 广东省科技计划重大专项 2017B020231001(2017.9-2020.8)子项目负责人

     题目:干细胞治疗的大动物模型及基于干细胞的动物嵌合技术平台建设

  1. 广东省科技计划重大专项 2014B020225007(2015.1-2017.12) 子项目负责人

     题目:基因敲除食蟹猴疾病模型的干细胞治疗研究

  1. 广州市科技计划项目(健康医疗协同创新重大专项) 201508020254(2014.11-2017.10) 子项目负责人

     题目:间充质干细胞治疗间质性肺疾病